LOCUS       XN21.GB      8917 BP DS-DNA   CIRCULAR  SYN       22-JAN-2007
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
FEATURES             Location/Qualifiers
     terminator      6555..6654
                     /note="TERM"
     rep_origin      complement(7153..7768)
                     /note="ColE1"
     rep_origin      2..1348
                     /note="2 micron"
     misc_recomb     4019..4143
                     /note="attR1"
     misc_recomb     6163..6287
                     /note="attR2"
     CDS             1782..2604
                     /note="TRP"
     CDS             4013..4018
                     /note="Mini-ORF"
     CDS             complement(4572..4877)
                     /note="ccdB"
     CDS             complement(5223..5882)
                     /note="CamR"
     CDS             6319..6429
                     /note="NubG"
     CDS             6433..6522
                     /note="HA-tag"
     CDS             complement(7930..8790)
                     /note="AmpR"
     rep_origin      complement(2661..3118)
                     /note="f1"
     promoter        3650..4008
                     /note="ADH"
BASE COUNT      2459 A      1968 C      1968 G      2522 T         0 OTHER
ORIGIN      -
        1 GAACGAAGCA TCTGTGCTTC ATTTTGTAGA ACAAAAATGC AACGCGAGAG CGCTAATTTT
       61 TCAAACAAAG AATCTGAGCT GCATTTTTAC AGAACAGAAA TGCAACGCGA AAGCGCTATT
      121 TTACCAACGA AGAATCTGTG CTTCATTTTT GTAAAACAAA AATGCAACGC GAGAGCGCTA
      181 ATTTTTCAAA CAAAGAATCT GAGCTGCATT TTTACAGAAC AGAAATGCAA CGCGAGAGCG
      241 CTATTTTACC AACAAAGAAT CTATACTTCT TTTTTGTTCT ACAAAAATGC ATCCCGAGAG
      301 CGCTATTTTT CTAACAAAGC ATCTTAGATT ACTTTTTTTC TCCTTTGTGC GCTCTATAAT
      361 GCAGTCTCTT GATAACTTTT TGCACTGTAG GTCCGTTAAG GTTAGAAGAA GGCTACTTTG
      421 GTGTCTATTT TCTCTTCCAT AAAAAAAGCC TGACTCCACT TCCCGCGTTT ACTGATTACT
      481 AGCGAAGCTG CGGGTGCATT TTTTCAAGAT AAAGGCATCC CCGATTATAT TCTATACCGA
      541 TGTGGATTGC GCATACTTTG TGAACAGAAA GTGATAGCGT TGATGATTCT TCATTGGTCA
      601 GAAAATTATG AACGGTTTCT TCTATTTTGT CTCTATATAC TACGTATAGG AAATGTTTAC
      661 ATTTTCGTAT TGTTTTCGAT TCACTCTATG AATAGTTCTT ACTACAATTT TTTTGTCTAA
      721 AGAGTAATAC TAGAGATAAA CATAAAAAAT GTAGAGGTCG AGTTTAGATG CAAGTTCAAG
      781 GAGCGAAAGG TGGATGGGTA GGTTATATAG GGATATAGCA CAGAGATATA TAGCAAAGAG
      841 ATACTTTTGA GCAATGTTTG TGGAAGCGGT ATTCGCAATA TTTTAGTAGC TCGTTACAGT
      901 CCGGTGCGTT TTTGGTTTTT TGAAAGTGCG TCTTCAGAGC GCTTTTGGTT TTCAAAAGCG
      961 CTCTGAAGTT CCTATACTTT CTAGAGAATA GGAACTTCGG AATAGGAACT TCAAAGCGTT
     1021 TCCGAAAACG AGCGCTTCCG AAAATGCAAC GCGAGCTGCG CACATACAGC TCACTGTTCA
     1081 CGTCGCACCT ATATCTGCGT GTTGCCTGTA TATATATATA CATGAGAAGA ACGGCATAGT
     1141 GCGTGTTTAT GCTTAAATGC GTACTTATAT GCGTCTATTT ATGTAGGATG AAAGGTAGTC
     1201 TAGTACCTCC TGTGATATTA TCCCATTCCA TGCGGGGTAT CGTATGCTTC CTTCAGCACT
     1261 ACCCTTTAGC TGTTCTATAT GCTGCCACTC CTCAATTGGA TTAGTCTCAT CCTTCAATGC
     1321 TATCATTTCC TTTGATATTG GATCATATTA AGAAACCATT ATTATCATGA CATTAACCTA
     1381 TAAAAATAGG CGTATCACGA GGCCCTTTCG TCTCGCGCGT TTCGGTGATG ACGGTGAAAA
     1441 CCTCTGACAC ATGCAGCTCC CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG
     1501 CAGACAAGCC CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT GGCTTAACTA
     1561 TGCGGCATCA GAGCAGATTG TACTGAGAGT GCACCATAGA TCAACGACAT TACTATATAT
     1621 ATAATATAGG AAGCATTTAA TAGACAGCAT CGTAATATAT GTGTACTTTG CAGTTATGAC
     1681 GCCAGATGGC AGTAGTGGAA GATATTCTTT ATTGAAAAAT AGCTTGTCAC CTTACGTACA
     1741 ATCTTGATCC GGAGCTTTTC TTTTTTTGCC GATTAAGAAT TAATTCGGTC GAAAAAAGAA
     1801 AAGGAGAGGG CCAAGAGGGA GGGCATTGGT GACTATTGAG CACGTGAGTA TACGTGATTA
     1861 AGCACACAAA GGCAGCTTGG AGTATGTCTG TTATTAATTT CACAGGTAGT TCTGGTCCAT
     1921 TGGTGAAAGT TTGCGGCTTG CAGAGCACAG AGGCCGCAGA ATGTGCTCTA GATTCCGATG
     1981 CTGACTTGCT GGGTATTATA TGTGTGCCCA ATAGAAAGAG AACAATTGAC CCGGTTATTG
     2041 CAAGGAAAAT TTCAAGTCTT GTAAAAGCAT ATAAAAATAG TTCAGGCACT CCGAAATACT
     2101 TGGTTGGCGT GTTTCGTAAT CAACCTAAGG AGGATGTTTT GGCTCTGGTC AATGATTACG
     2161 GCATTGATAT CGTCCAACTG CATGGAGATG AGTCGTGGCA AGAATACCAA GAGTTCCTCG
     2221 GTTTGCCAGT TATTAAAAGA CTCGTATTTC CAAAAGACTG CAACATACTA CTCAGTGCAG
     2281 CTTCACAGAA ACCTCATTCG TTTATTCCCT TGTTTGATTC AGAAGCAGGT GGGACAGGTG
     2341 AACTTTTGGA TTGGAACTCG ATTTCTGACT GGGTTGGAAG GCAAGAGAGC CCCGAAAGCT
     2401 TACATTTTAT GTTAGCTGGT GGACTGACGC CAGAAAATGT TGGTGATGCG CTTAGATTAA
     2461 ATGGCGTTAT TGGTGTTGAT GTAAGCGGAG GTGTGGAGAC AAATGGTGTA AAAGACTCTA
     2521 ACAAAATAGC AAATTTCGTC AAAAATGCTA AGAAATAGGT TATTACTGAG TAGTATTTAT
     2581 TTAAGTATTG TTTGTGCACT TGCCGATCTA TGCGGTGTGA AATACCGCAC AGATGCGTAA
     2641 GGAGAAAATA CCGCATCAGG AAATTGTAAA CGTTAATATT TTGTTAAAAT TCGCGTTAAA
     2701 TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA
     2761 ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT
     2821 ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC
     2881 ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA
     2941 TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC
     3001 GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT
     3061 CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCGCG
     3121 CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT
     3181 ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG TAACGCCAGG
     3241 GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTCGT CCAAGCTTTC GCGAGCTCGA
     3301 TATCCTTTTG TTGTTTCCGG GTGTACAATA TGGACTTCCT CTTTTCTGGC AACCAAACCC
     3361 ATACATCGGG ATTCCTATAA TACCTTCGTT GGTCTCCCTA ACATGTAGGT GGCGGAGGGG
     3421 AGATATACAA TAGAACAGAT ACCAGACAAG ACATAATGGG CTAAACAAGA CTACACCAAT
     3481 TACACTGCCT CATTGATGGT GGTACATAAC GAACTAATAC TGTAGCCCTA GACTTGATAG
     3541 CCATCATCAT ATCGAAGTTT CACTACCCTT TTTCCATTTG CCATCTATTG AAGTAATAAT
     3601 AGGCGCATGC AACTTCTTTT CTTTTTTTTT CTTTTCTCTC TCCCCCGTTG TTGTCTCACC
     3661 ATATCCGCAA TGACAAAAAA AATGATGGAA GACACTAAAG GAAAAAATTA ACGACAAAGA
     3721 CAGCACCAAC AGATGTCGTT GTTCCAGAGC TGATGAGGGG TATCTTCGAA CACACGAAAC
     3781 TTTTTCCTTC CTTCATTCAC GCACACTACT CTCTAATGAG CAACGGTATA CGGCCTTCCT
     3841 TCCAGTTACT TGAATTTGAA ATAAAAAAAG TTTGCCGCTT TGCTATCAAG TATAAATAGA
     3901 CCTGCAATTA TTAATCTTTT GTTTCCTCGT CATTGTTCTC GTTCCCTTTC TTCCTTGTTT
     3961 CTTTTTTCTG CACAATATTT CAAGCTATAC CAAGCATACA ATCAACTCAT ATGTGAACAA
     4021 GTTTGTACAA AAAAGCTGAA CGAGAAACGT AAAATGATAT AAATATCAAT ATATTAAATT
     4081 AGATTTTGCA TAAAAAACAG ACTACATAAT ACTGTAAAAC ACAACATATC CAGTCACTAT
     4141 GAATCAACTA CTTAGATGGT ATTAGTGACC TGTAGTCGAC CGACAGCCTT CCAAATGTTC
     4201 TTCGGGTGAT GCTGCCAACT TAGTCGACCG ACAGCCTTCC AAATGTTCTT CTCAAACGGA
     4261 ATCGTCGTAT CCAGCCTACT CGCTATTGTC CTCAATGCCG TATTAAATCA TAAAAAGAAA
     4321 TAAGAAAAAG AGGTGCGAGC CTCTTTTTTG TGTGACAAAA TAAAAACATC TACCTATTCA
     4381 TATACGCTAG TGTCATAGTC CTGAAAATCA TCTGCATCAA GAACAATTTC ACAACTCTTA
     4441 TACTTTTCTC TTACAAGTCG TTCGGCTTCA TCTGGATTTT CAGCCTCTAT ACTTACTAAA
     4501 CGTGATAAAG TTTCTGTAAT TTCTACTGTA TCGACCTGCA GACTGGCTGT GTATAAGGGA
     4561 GCCTGACATT TATATTCCCC AGAACATCAG GTTAATGGCG TTTTTGATGT CATTTTCGCG
     4621 GTGGCTGAGA TCAGCCACTT CTTCCCCGAT AACGGAGACC GGCACACTGG CCATATCGGT
     4681 GGTCATCATG CGCCAGCTTT CATCCCCGAT ATGCACCACC GGGTAAAGTT CACGGGAGAC
     4741 TTTATCTGAC AGCAGACGTG CACTGGCCAG GGGGATCACC ATCCGTCGCC CGGGCGTGTC
     4801 AATAATATCA CTCTGTACAT CCACAAACAG ACGATAACGG CTCTCTCTTT TATAGGTGTA
     4861 AACCTTAAAC TGCATTTCAC CAGCCCCTGT TCTCGTCAGC AAAAGAGCCG TTCATTTCAA
     4921 TAAACCGGGC GACCTCAGCC ATCCCTTCCT GATTTTCCGC TTTCCAGCGT TCGGCACGCA
     4981 GACGACGGGC TTCATTCTGC ATGGTTGTGC TTACCAGACC GGAGATATTG ACATCATATA
     5041 TGCCTTGAGC AACTGATAGC TGTCGCTGTC AACTGTCACT GTAATACGCT GCTTCATAGC
     5101 ATACCTCTTT TTGACATACT TCGGGTATAC ATATCAGTAT ATATTCTTAT ACCGCAAAAA
     5161 TCAGCGCGCA AATACGCATA CTGTTATCTG GCTTTTAGTA AGCCGGATCC ACGCGGCGTT
     5221 TACGCCCCGC CCTGCCACTC ATCGCAGTAC TGTTGTAATT CATTAAGCAT TCTGCCGACA
     5281 TGGAAGCCAT CACAGACGGC ATGATGAACC TGAATCGCCA GCGGCATCAG CACCTTGTCG
     5341 CCTTGCGTAT AATATTTGCC CATGGTGAAA ACGGGGGCGA AGAAGTTGTC CATATTGGCC
     5401 ACGTTTAAAT CAAAACTGGT GAAACTCACC CAGGGATTGG CTGAGACGAA AAACATATTC
     5461 TCAATAAACC CTTTAGGGAA ATAGGCCAGG TTTTCACCGT AACACGCCAC ATCTTGCGAA
     5521 TATATGTGTA GAAACTGCCG GAAATCGTCG TGGTATTCAC TCCAGAGCGA TGAAAACGTT
     5581 TCAGTTTGCT CATGGAAAAC GGTGTAACAA GGGTGAACAC TATCCCATAT CACCAGCTCA
     5641 CCGTCTTTCA TTGCCATACG GAATTCCGGA TGAGCATTCA TCAGGCGGGC AAGAATGTGA
     5701 ATAAAGGCCG GATAAAACTT GTGCTTATTT TTCTTTACGG TCTTTAAAAA GGCCGTAATA
     5761 TCCAGCTGAA CGGTCTGGTT ATAGGTACAT TGAGCAACTG ACTGAAATGC CTCAAAATGT
     5821 TCTTTACGAT GCCATTGGGA TATATCAACG GTGGTATATC CAGTGATTTT TTTCTCCATT
     5881 TTAGCTTCCT TAGCTCCTGA AAATCTCGAT AACTCAAAAA ATACGCCCGG TAGTGATCTT
     5941 ATTTCATTAT GGTGAAAGTT GGAACCTCTT ACGTGCCGAT CAACGTCTCA TTTTCGCCAA
     6001 AAGTTGGCCC AGGGCTTCCC GGTATCAACA GGGACACCAG GATTTATTTA TTCTGCGAAG
     6061 TGATCTTCCG TCACAGGTAT TTATTCGGCG CAAAGTGCGT CGGGTGATGC TGCCAACTTA
     6121 GTCGACTACA GGTCACTAAT ACCATCTAAG TAGTTGATTC ATAGTGACTG GATATGTTGT
     6181 GTTTTACAGT ATTATGTAGT CTGTTTTTTA TGCAAAATCT AATTTAATAT ATTGATATTT
     6241 ATATCATTTT ACGTTTCTCG TTCAGCTTTC TTGTACAAAG TGGTTGGTGG TGGCGGATCC
     6301 GGTGGAGGTG GATCAATGCA GATTTTCGTC AAGACTTTGA CCGGTAAAAC CGGAACATTG
     6361 GAAGTTGAAT CTTCCGATAC CATCGACAAC GTTAAGTCGA AAATTCAAGA CAAGGAAGGA
     6421 ATCCCTGGTT ACCCATACGA TGTTCCTGAC TATGCGGGCT ATCCCTATGA CGTCCCGGAC
     6481 TATGCAGGAT CCTATCCATA TGACGTTCCA GATTACGCTG CTCAGTGCTG AGGCGCGCCA
     6541 CTTCTAAATA AGCGAATTTC TTATGATTTA TGATTTTTAT TATTAAATAA GTTATAAAAA
     6601 AAATAAGTGT ATACAAATTT TAAAGTGACT CTTAGGTTTT AAAACGAAAA TTCTTATTCT
     6661 TGAGTAACTC TTTCCTGTAG GTCAGGTTGC TTTCTCAGGT ATAGTATGAG GTCGCTCTTA
     6721 TTGACCACAC CTCTACCGGC AGCATGCAAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC
     6781 TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG
     6841 TAAAGCCTGG GGTGCCTAAT GAGTGAGGTA ACTCACATTA ATTGCGTTGC GCTCACTGCC
     6901 CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGGATTAA TGAATCGGCC AACGCGCGGG
     6961 GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC
     7021 GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC
     7081 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA
     7141 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA
     7201 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
     7261 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
     7321 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA
     7381 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
     7441 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA
     7501 CTTATCGCCA CTGGCAGCAG CCACTAGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
     7561 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG
     7621 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
     7681 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
     7741 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA
     7801 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT
     7861 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC
     7921 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC
     7981 ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC
     8041 TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC
     8101 AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC
     8161 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT
     8221 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC
     8281 TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA
     8341 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT
     8401 ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG
     8461 CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC
     8521 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA
     8581 AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT
     8641 GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT
     8701 CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG
     8761 GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA
     8821 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT
     8881 AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCT
//
