LOCUS NX32.GB 8899 BP DS-DNA SYN 01-FEB-2007 DEFINITION - ACCESSION - KEYWORDS - SOURCE - FEATURES Location/Qualifiers rep_origin 1..1348 /note="2 micron" CDS 1782..2604 /note="TRP1" rep_origin complement(2661..3118) /note="f1 ori" promoter 3650..4008 /note="ADH" CDS 4013..4123 /note="NubG" misc_recomb 4133..4257 /note="attR1" misc_recomb 6276..6400 /note="attR2" CDS complement(4686..4991) /note="ccdB" CDS complement(5336..5995) /note="CamR" misc_feature 6402..6413 /note="linker" misc_feature 6414..6503 /note="HA-tag" terminator 6536..6726 /note="ADH" rep_origin 7135..7750 /note="ColE1" CDS complement(7912..8772) /note="AmpR" BASE COUNT 2454 A 1965 C 1965 G 2515 T 0 OTHER ORIGIN - 1 GAACGAAGCA TCTGTGCTTC ATTTTGTAGA ACAAAAATGC AACGCGAGAG CGCTAATTTT 61 TCAAACAAAG AATCTGAGCT GCATTTTTAC AGAACAGAAA TGCAACGCGA AAGCGCTATT 121 TTACCAACGA AGAATCTGTG CTTCATTTTT GTAAAACAAA AATGCAACGC GAGAGCGCTA 181 ATTTTTCAAA CAAAGAATCT GAGCTGCATT TTTACAGAAC AGAAATGCAA CGCGAGAGCG 241 CTATTTTACC AACAAAGAAT CTATACTTCT TTTTTGTTCT ACAAAAATGC ATCCCGAGAG 301 CGCTATTTTT CTAACAAAGC ATCTTAGATT ACTTTTTTTC TCCTTTGTGC GCTCTATAAT 361 GCAGTCTCTT GATAACTTTT TGCACTGTAG GTCCGTTAAG GTTAGAAGAA GGCTACTTTG 421 GTGTCTATTT TCTCTTCCAT AAAAAAAGCC TGACTCCACT TCCCGCGTTT ACTGATTACT 481 AGCGAAGCTG CGGGTGCATT TTTTCAAGAT AAAGGCATCC CCGATTATAT TCTATACCGA 541 TGTGGATTGC GCATACTTTG TGAACAGAAA GTGATAGCGT TGATGATTCT TCATTGGTCA 601 GAAAATTATG AACGGTTTCT TCTATTTTGT CTCTATATAC TACGTATAGG AAATGTTTAC 661 ATTTTCGTAT TGTTTTCGAT TCACTCTATG AATAGTTCTT ACTACAATTT TTTTGTCTAA 721 AGAGTAATAC TAGAGATAAA CATAAAAAAT GTAGAGGTCG AGTTTAGATG CAAGTTCAAG 781 GAGCGAAAGG TGGATGGGTA GGTTATATAG GGATATAGCA CAGAGATATA TAGCAAAGAG 841 ATACTTTTGA GCAATGTTTG TGGAAGCGGT ATTCGCAATA TTTTAGTAGC TCGTTACAGT 901 CCGGTGCGTT TTTGGTTTTT TGAAAGTGCG TCTTCAGAGC GCTTTTGGTT TTCAAAAGCG 961 CTCTGAAGTT CCTATACTTT CTAGAGAATA GGAACTTCGG AATAGGAACT TCAAAGCGTT 1021 TCCGAAAACG AGCGCTTCCG AAAATGCAAC GCGAGCTGCG CACATACAGC TCACTGTTCA 1081 CGTCGCACCT ATATCTGCGT GTTGCCTGTA TATATATATA CATGAGAAGA ACGGCATAGT 1141 GCGTGTTTAT GCTTAAATGC GTACTTATAT GCGTCTATTT ATGTAGGATG AAAGGTAGTC 1201 TAGTACCTCC TGTGATATTA TCCCATTCCA TGCGGGGTAT CGTATGCTTC CTTCAGCACT 1261 ACCCTTTAGC TGTTCTATAT GCTGCCACTC CTCAATTGGA TTAGTCTCAT CCTTCAATGC 1321 TATCATTTCC TTTGATATTG GATCATATTA AGAAACCATT ATTATCATGA CATTAACCTA 1381 TAAAAATAGG CGTATCACGA GGCCCTTTCG TCTCGCGCGT TTCGGTGATG ACGGTGAAAA 1441 CCTCTGACAC ATGCAGCTCC CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG 1501 CAGACAAGCC CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT GGCTTAACTA 1561 TGCGGCATCA GAGCAGATTG TACTGAGAGT GCACCATAGA TCAACGACAT TACTATATAT 1621 ATAATATAGG AAGCATTTAA TAGACAGCAT CGTAATATAT GTGTACTTTG CAGTTATGAC 1681 GCCAGATGGC AGTAGTGGAA GATATTCTTT ATTGAAAAAT AGCTTGTCAC CTTACGTACA 1741 ATCTTGATCC GGAGCTTTTC TTTTTTTGCC GATTAAGAAT TAATTCGGTC GAAAAAAGAA 1801 AAGGAGAGGG CCAAGAGGGA GGGCATTGGT GACTATTGAG CACGTGAGTA TACGTGATTA 1861 AGCACACAAA GGCAGCTTGG AGTATGTCTG TTATTAATTT CACAGGTAGT TCTGGTCCAT 1921 TGGTGAAAGT TTGCGGCTTG CAGAGCACAG AGGCCGCAGA ATGTGCTCTA GATTCCGATG 1981 CTGACTTGCT GGGTATTATA TGTGTGCCCA ATAGAAAGAG AACAATTGAC CCGGTTATTG 2041 CAAGGAAAAT TTCAAGTCTT GTAAAAGCAT ATAAAAATAG TTCAGGCACT CCGAAATACT 2101 TGGTTGGCGT GTTTCGTAAT CAACCTAAGG AGGATGTTTT GGCTCTGGTC AATGATTACG 2161 GCATTGATAT CGTCCAACTG CATGGAGATG AGTCGTGGCA AGAATACCAA GAGTTCCTCG 2221 GTTTGCCAGT TATTAAAAGA CTCGTATTTC CAAAAGACTG CAACATACTA CTCAGTGCAG 2281 CTTCACAGAA ACCTCATTCG TTTATTCCCT TGTTTGATTC AGAAGCAGGT GGGACAGGTG 2341 AACTTTTGGA TTGGAACTCG ATTTCTGACT GGGTTGGAAG GCAAGAGAGC CCCGAAAGCT 2401 TACATTTTAT GTTAGCTGGT GGACTGACGC CAGAAAATGT TGGTGATGCG CTTAGATTAA 2461 ATGGCGTTAT TGGTGTTGAT GTAAGCGGAG GTGTGGAGAC AAATGGTGTA AAAGACTCTA 2521 ACAAAATAGC AAATTTCGTC AAAAATGCTA AGAAATAGGT TATTACTGAG TAGTATTTAT 2581 TTAAGTATTG TTTGTGCACT TGCCGATCTA TGCGGTGTGA AATACCGCAC AGATGCGTAA 2641 GGAGAAAATA CCGCATCAGG AAATTGTAAA CGTTAATATT TTGTTAAAAT TCGCGTTAAA 2701 TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA 2761 ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT 2821 ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC 2881 ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA 2941 TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC 3001 GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT 3061 CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCGCG 3121 CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT 3181 ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG TAACGCCAGG 3241 GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTCGT CCAAGCTTTC GCGAGCTCGA 3301 TATCCTTTTG TTGTTTCCGG GTGTACAATA TGGACTTCCT CTTTTCTGGC AACCAAACCC 3361 ATACATCGGG ATTCCTATAA TACCTTCGTT GGTCTCCCTA ACATGTAGGT GGCGGAGGGG 3421 AGATATACAA TAGAACAGAT ACCAGACAAG ACATAATGGG CTAAACAAGA CTACACCAAT 3481 TACACTGCCT CATTGATGGT GGTACATAAC GAACTAATAC TGTAGCCCTA GACTTGATAG 3541 CCATCATCAT ATCGAAGTTT CACTACCCTT TTTCCATTTG CCATCTATTG AAGTAATAAT 3601 AGGCGCATGC AACTTCTTTT CTTTTTTTTT CTTTTCTCTC TCCCCCGTTG TTGTCTCACC 3661 ATATCCGCAA TGACAAAAAA AATGATGGAA GACACTAAAG GAAAAAATTA ACGACAAAGA 3721 CAGCACCAAC AGATGTCGTT GTTCCAGAGC TGATGAGGGG TATCTTCGAA CACACGAAAC 3781 TTTTTCCTTC CTTCATTCAC GCACACTACT CTCTAATGAG CAACGGTATA CGGCCTTCCT 3841 TCCAGTTACT TGAATTTGAA ATAAAAAAAG TTTGCCGCTT TGCTATCAAG TATAAATAGA 3901 CCTGCAATTA TTAATCTTTT GTTTCCTCGT CATTGTTCTC GTTCCCTTTC TTCCTTGTTT 3961 CTTTTTCTGC ACAATATTTC AAGCTATACC AAGCATACAA TCAACTCGAG ATGCAGATTT 4021 TCGTCAAGAC TTTGACCGGT AAAACCGGAA CATTGGAAGT TGAATCTTCC GATACCATCG 4081 ACAACGTTAA GTCGAAAATT CAAGACAAGG AAGGAATCCC TGGTGGGTCG ACAAGTTTGT 4141 ACAAAAAAGC TGAACGAGAA ACGTAAAATG ATATAAATAT CAATATATTA AATTAGATTT 4201 TGCATAAAAA ACAGACTACA TAATACTGTA AAACACAACA TATCCAGTCA CTATGAATCA 4261 ACTACTTAGA TGGTATTAGT GACCTGTAGT CGACCGACAG CCTTCCAAAT GTTCTTCGGG 4321 TGATGCTGCC AACTTAGTCG ACCGACAGCC TTCCAAATGT TCTTCTCAAA CGGAATCGTC 4381 GTATCCAGCC TACTCGCTAT TGTCCTCAAT GCCGTATTAA ATCATAAAAA GAAATAAGAA 4441 AAAGAGGTGC GAGCCTCTTT TTTGTGTGAC AAAATAAAAA CATCTACCTA TTCATATACG 4501 CTAGTGTCAT AGTCCTGAAA ATCATCTGCA TCAAGAACAA TTTCACAACT CTTATACTTT 4561 TCTCTTACAA GTCGTTCGGC TTCATCTGGA TTTTCAGCCT CTATACTTAC TAAACGTGAT 4621 AAAGTTTCTG TAATTTCTAC TGTATCGACC TGCAGACTGG CTGTGTATAA GGGAGCCTGA 4681 CATTTATATT CCCCAGAACA TCAGGTTAAT GGCGTTTTTG ATGTCATTTT CGCGGTGGCT 4741 GAGATCAGCC ACTTCTTCCC CGATAACGGA GACCGGCACA CTGGCCATAT CGGTGGTCAT 4801 CATGCGCCAG CTTTCATCCC CGATATGCAC CACCGGGTAA AGTTCACGGG AGACTTTATC 4861 TGACAGCAGA CGTGCACTGG CCAGGGGGAT CACCATCCGT CGCCCGGGCG TGTCAATAAT 4921 ATCACTCTGT ACATCCACAA ACAGACGATA ACGGCTCTCT CTTTTATAGG TGTAAACCTT 4981 AAACTGCATT TCACCAGCCC CTGTTCTCGT CAGCAAAAGA GCCGTTCATT TCAATAAACC 5041 GGGCGACCTC AGCCATCCCT TCCTGATTTT CCGCTTTCCA GCGTTCGGCA CGCAGACGAC 5101 GGGCTTCATT CTGCATGGTT GTGCTTACCA GACCGGAGAT ATTGACATCA TATATGCCTT 5161 GAGCAACTGA TAGCTGTCGC TGTCAACTGT CACTGTAATA CGCTGCTTCA TAGCATACCT 5221 CTTTTTGACA TACTTCGGGT ATACATATCA GTATATATTC TTATACCGCA AAAATCAGCG 5281 CGCAAATACG CATACTGTTA TCTGGCTTTT AGTAAGCCGG ATCCACGCGG CGTTTACGCC 5341 CCGCCCTGCC ACTCATCGCA GTACTGTTGT AATTCATTAA GCATTCTGCC GACATGGAAG 5401 CCATCACAGA CGGCATGATG AACCTGAATC GCCAGCGGCA TCAGCACCTT GTCGCCTTGC 5461 GTATAATATT TGCCCATGGT GAAAACGGGG GCGAAGAAGT TGTCCATATT GGCCACGTTT 5521 AAATCAAAAC TGGTGAAACT CACCCAGGGA TTGGCTGAGA CGAAAAACAT ATTCTCAATA 5581 AACCCTTTAG GGAAATAGGC CAGGTTTTCA CCGTAACACG CCACATCTTG CGAATATATG 5641 TGTAGAAACT GCCGGAAATC GTCGTGGTAT TCACTCCAGA GCGATGAAAA CGTTTCAGTT 5701 TGCTCATGGA AAACGGTGTA ACAAGGGTGA ACACTATCCC ATATCACCAG CTCACCGTCT 5761 TTCATTGCCA TACGGAATTC CGGATGAGCA TTCATCAGGC GGGCAAGAAT GTGAATAAAG 5821 GCCGGATAAA ACTTGTGCTT ATTTTTCTTT ACGGTCTTTA AAAAGGCCGT AATATCCAGC 5881 TGAACGGTCT GGTTATAGGT ACATTGAGCA ACTGACTGAA ATGCCTCAAA ATGTTCTTTA 5941 CGATGCCATT GGGATATATC AACGGTGGTA TATCCAGTGA TTTTTTTCTC CATTTTAGCT 6001 TCCTTAGCTC CTGAAAATCT CGATAACTCA AAAAATACGC CCGGTAGTGA TCTTATTTCA 6061 TTATGGTGAA AGTTGGAACC TCTTACGTGC CGATCAACGT CTCATTTTCG CCAAAAGTTG 6121 GCCCAGGGCT TCCCGGTATC AACAGGGACA CCAGGATTTA TTTATTCTGC GAAGTGATCT 6181 TCCGTCACAG GTATTTATTC GGCGCAAAGT GCGTCGGGTG ATGCTGCCAA CTTAGTCGAC 6241 TACAGGTCAC TAATACCATC TAAGTAGTTG ATTCATAGTG ACTGGATATG TTGTGTTTTA 6301 CAGTATTATG TAGTCTGTTT TTTATGCAAA ATCTAATTTA ATATATTGAT ATTTATATCA 6361 TTTTACGTTT CTCGTTCAGC TTTCTTGTAC AAAGTGGTTG GTGGTGGCGG ATACCCATAC 6421 GATGTTCCTG ACTATGCGGG CTATCCGTAT GACGTCCCGG ACTATGCAGG ATCCTATCCA 6481 TATGACGTTC CAGATTACGC TGCTCAGTGC TGAGGCGCGC CACTTCTAAA TAAGCGAATT 6541 TCTTATGATT TATGATTTTT ATTATTAAAT AAGTTATAAA AAAAATAAGT GTATACAAAT 6601 TTTAAAGTGA CTCTTAGGTT TTAAAACGAA AATTCTTATT CTTGAGTAAC TCTTTCCTGT 6661 AGGTCAGGTT GCTTTCTCAG GTATAGTATG AGGTCGCTCT TATTGACCAC ACCTCTACCG 6721 GCAGCATGCA AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC 6781 GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA 6841 ATGAGTGAGG TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA 6901 CCTGTCGTGC CAGCTGGATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT 6961 TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG 7021 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 7081 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 7141 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 7201 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 7261 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 7321 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 7381 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 7441 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 7501 AGCCACTAGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 7561 GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA 7621 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 7681 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 7741 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 7801 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 7861 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT 7921 AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT 7981 CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT 8041 GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG 8101 AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG 8161 TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT 8221 TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC 8281 CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT 8341 CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC 8401 AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA 8461 GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC 8521 GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA 8581 ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA 8641 ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG 8701 AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG 8761 AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT 8821 GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT 8881 TCCCCGAAAA GTGCCACCT //